Department of Cell Biology


Research Publications    Education Publications    Older Publications

Research Publications

"Substrate identification and specificity profiling of deubiquitylases against endogenously-generated ubiquitin-protein conjugates" V. Rossio, J.A. Paulo, X. Liu, S.P. Gygi, R.W. King 2023 BioRxiv

"Identification of deubiquitinase substrates in Xenopus egg extract" V. Rossio, J.A. Paulo, R.W. King. Methods Mol Biol. 2023 2591:219-236. Pubmed

"Proteomics of broad deubiquitylase inhibition unmasks redundant enzyme function to reveal substrates and assess enzyme specificity" V. Rossio, J.A. Paulo, J. Chick, B. Brasher, S.P. Gygi, R.W. King Cell Chem Biol 2021 28, 1-16. Pubmed

"The insulin receptor adaptor IRS2 is an APC/C substrate that promotes cell cycle protein expression and a robust spindle assembly checkpoint" S. Manohar, Q. Yu, S.P. Gygi, R.W. King Mol Cell Proteomics 2020 Pubmed

"Paradoxical mitotic exit induced by a small molecule inhibitor of APC/CCdc20" K.V. Richeson, T. Bodrug, K.L. Sackton, M. Yamaguchi, J.A. Paulo, S.P. Gygi, B.A. Schulman, N.G. Brown, R.W. King Nat Chem Biol 2020 16:546-555. Pubmed

"Amplifiers co-translationally enhance CFTR biosynthesis via PCBP1-mediated regulation of CFTR mRNA" D. Dukovski, A. Villella, C. Bastos, R. King, D. Finley, J.W. Kelly, R.I. Morimoto, F.U. Hartl, B. Munoz, P.S. Lee, M. Zecevic, J.P. Miller J Cyst Fibros 2020 doi: 10.1016/j.jcf.2020.02.006 Pubmed

"An inhibitor of the proteasomal deubiquitinating enzyme USP14 induces tau elimination in cultured neurons" M. Boselli, B.H. Lee, J. Robert, M. Prado, S. Min, C. Cheng, M.C. Silva, C. Seong, S. Elsasser, K.M. Hatle, T.C. Gahman, S.P. Gygi, S.J. Haggarty, L. Gan, R.W. King, D. Finley J Biol Chem 2017  292: 19209-19225. Pubmed

"USP14 deubiquitinates proteasome-bound substrates that are ubiquitinated at multiple sites" B.H. Lee , Y. Lu, M.A. Prado, Y. Shi, G. Tian, S. Sun, S. Elsasser, S.P. Gygi, R.W. King, D. Finley. Nature 2016; 532:398-401. Pubmed

"Inhibiting the anaphase promoting complex/cyclosome induces a metaphase arrest and cell death in multiple myeloma cells"  S. Lub, A. Maes, K. Maes, K. De Veirman, E. De Bruyne, E. Menu, K. Fostier, A. Kassambara, J. Moreaux, D. Hose, X. Leleu, R.W. King, K. Vanderkerken, E. Van Valckenborgh. Oncotarget 2016; 7:4062-76. Pubmed

"Defective sister chromatid cohesion is synthetically lethal with impaired APC/C function" J. de Lange, A. Faramarz, A.B. Oostra, R.X. de Menezes, I.H. van der Meulen, M. A. Rooimans, D.A. Rockx, R.H. Brakenhoff, V.W. van Beusechem, R.W. King, J.P. de Winter, R.M. Wolthuis. Nature Communications 2015; 6:8399.

"Substrate degradation by the proteasome: a single-molecule kinetic analysis" Lu Y, Lee BH, King RW, Finley D, Kirschner MW. Science 2015; 348(6231):1250834. Pubmed

"Synergistic blockade of mitotic exit by two chemical inhibitors of the APC/C" K.L. Sackton, N. Dimova, X. Zeng , W. Tian, M. Zhang , T.B. Sackton, J. Meaders, K.L. Pfaff, F. Sigoillot, H. Yu, X Luo, R.W. King Nature  2014 ,514: 646-9. Pubmed

"Sculpting the proteome with small molecules" R.W. King and D. Finley Nature Chemical Biology 2014, 10(11):870-4. Pubmed

"Determinants of human cyclin B1 association with mitotic chromosomes" K.L. Pfaff and R.W. King PLoS ONE 2013 8(3):e59169. PLoS ONE

"The G2/M regulator histone demethylase PHF8 is targeted for degradation by the anaphase-promoting complex containing CDC20" H.J. Lim, N.V. Dimova, M.K. Tan, F. Sigoillot, R.W. King, Y. Shi Mol. Cell. Biol. 2013 33:4166-80. Pubmed

"A high-throughput screening method for identification of inhibitors of the deubiquitinating enzyme Usp14" B.H. Lee, D. Finley, R.W. King Curr Protoc Chem Biol 2012 4:311-30. Pubmed

"An APC/C inhibitor stabilizes cyclin B1 by prematurely terminating ubiquitination" X. Zeng and R.W. King Nature Chemical Biology 2012 Feb 26. Pubmed

"A bioinformatics method identifies prominent off-targeted transcripts in RNAi screens" F.D. Sigoillot, S. Lyman, J.F. Huckins, B. Adamson, E. Chung, B. Quattrochi, R.W. King. Nature Methods 2012 Feb 19. Pubmed Supplementary program files

"A genome-wide homologous recombination screen identifies the RNA-binding protein RBMX as a component of the DNA-damage response" B. Adamson, A. Smogorzewska, F.D. Sigoillot, R.W. King, S.J. Elledge. Nature Cell Biology 2012 Feb 19;14(3):318-28. Pubmed

"APC/C-mediated multiple monoubiquitylation provides an alternative degradation signal for cyclin B1" N.V. Dimova, N.A. Hathaway, B.H. Lee, D.S. Kirkpatrick, M.L. Berkowitz, S.P. Gygi, D. Finley, R.W. King Nature Cell Biol. 2012, 14:168-176. Pubmed

"A Time-series method for automated measurement of changes in mitotic and interphase duration from time-lapse movies" F.D. Sigoillot, J.F. Huckins, F. Li, X. Zhou, S.T.C. Wong, R.W. King PLoS ONE 2011, 6(9): e25511. PLoS ONE 

"Stuck in the middle: Drugging the ubiquitin system at the E2 step" J.W. Harper and R.W. King Cell 2011, 145:1007-9.

"High-throughput kinase profiling: a more efficient approach toward the discovery of new kinase inhibitors" C.V. Miduturu, X. Deng, N. Kwiatkowski, W. Yang, L. Brault, P. Filippakopoulos, E. Chung, Q. Yang, J. Schwaller, S. Knapp, R.W. King, J.D. Lee, S. Herrgard, P. Zarrinkar, N.S. Gray Chem Biol. 2011 18:868-79. Pubmed

"Vigilance and validation: Keys to succes in RNAi screening" F.D. Sigoillot, R.W. King ACS Chemical Biology 2011, 6:47-60. Pubmed

"A non-genetic route to aneuploidy in human cancers" M. Krajcovic, N.B. Johnson, Q. Sun, G. Normand, N. Hoover, E. Yao, A.L. Richardson, R.W. King, E.S. Cibas, S.J. Schnitt, J.S. Brugge, M. Overholtzer Nature Cell Biology 2011, 13: 324-330. Pubmed

"Pharmacologic inhibition of the anaphase-promoting complex induces a spindle checkpoint-dependent mitotic arrest in the absence of spindle damage" X. Zeng, F. Sigoillot, S. Gaur, S. Choi, K.L. Pfaff, D.C. Oh, N. Hathaway, N. Dimova, G.D. Cuny, R.W. King Cancer Cell 2010, 18: 382-95. Pubmed

"Trimming of ubiquitin chains by proteasome-associated deubiquitinating enzymes" M.J. Lee, B.H. Lee, J. Hanna, R.W. King, D. Finley Mol Cell Proteomics 2010 doi:10.1074/mcp.R110.003871  Pubmed

"Enhancement of proteasome activity by a small-molecule inhibitor of USP14" B.H. Lee, M.J. Lee, S. Park, D.C. Oh, S. Elsasser, P.C. Chen, C. Gartner, N. Dimova, J. Hanna, S.P. Gygi, S.M. Wilson, R.W. King, D. Finley Nature 2010, 467:179-84. Pubmed

"Understanding cytokinesis failure" G. Normand, R.W. King Adv Exp Med Biol. 2010 676:27-55. Pubmed

"When 2+2=5: the origins and fates of aneuploid and tetraploid cells" R.W. King Biochem Biophys Acta 2008 1786:4-14.  Pubmed

"Novel cell segmentation and online SVM for cell cycle phase identification in automated microscopy" M. Wang, X. Zhou, F. Li, J. Huckins, R.W. King, S.M Wong Bioinformatics 2008 24:94-101. Pubmed

"Distinct sequence elements of cyclin B1 promote localization to chromatin, centrosomes and kinetochores during mitosis" A. Bentley, G. Normand, J. Hoyt, R.W. King Mol. Biol. Cell, 2007, 18: 4847-4858. Pubmed

"A nonapoptotic cell death process, entosis, that occurs by cell-in-cell invasion", M. Overholtzer, A. A. Mailleux, G. Mouneimne, G. Normand, S.J. Schnitt, R.W. King, E.S. Cibas and J.S. Brugge Cell, 2007 131: 966-979. Pubmed

"Stability of the proteasome can be regulated allosterically through engagement of its proteolytic active sites" M.F. Kleijnen, J. Roelofs, S. Park, N.A. Hathaway, M. Glickman, R.W. King, D. Finley Nat. Struct. Mol. Biol. 2007, In press. Pubmed 

"Context based mixture model for cell phase identification in automated fluorescence microscopy" M. Wang, X. Zhou, R.W. King and S.T. Wong BMC Bioinformatics 2007 30: 8-32. Pubmed

"Long-term imaging of mammalian cells using wide-field microscopy" M. Bentley, R. King J Vis Exp 2006 1:120. Pubmed

"Quantitative analysis of in vitro ubiquitinated cyclin B1 reveals complex chain topology" D.S. Kirkpatrick*, N.A. Hathaway*, J. Hanna, S. Elsasser, J. Rush, D. Finley, R.W. King, and S.P. Gygi Nature Cell Biology, 2006 8:700-710. *Co-first authors Co-corresponding authors Pubmed

"Deubiquitinating enzyme Ubp6 functions noncatalytically to delay proteasomal degredation" J. Hanna, N.A. Hathaway, Y. Tone, S. Elsasser, B. Crosas,  D.S. Kirkpatrick,  D.S. Leggett,  S.P. Gygi, R.W. King, and D. Finley Cell, 2006 127: 99-111. Pubmed

"Ubiquitin chains are remodeled at the proteasome by opposing ubiquitin ligase and deubiquitinating activities" B. Crosas, J. Hanna, D.S. Kirkpatrick, D.P. Zhang, Y. Tone, N.A. Hathaway, C. Buecker, D.S. Leggett, M. Schmidt, R.W. King, S.P. Gygi, and D. Finley Cell 2006 127: 1401-13. Pubmed

"Chromosome nondisjunction yields tetraploid rather than aneuploid cells in human cell lines" Q. Shi and R.W. King  Nature 2005 437:1038-42. Pubmed  

"Identifying small molecule inhibitors of the ubiquitin-proteasome pathway in Xenopus egg extracts" Salic, A. and King, R.W. Meth Enzymol, 2005399:576-585. Pubmed

"Small molecules that delay S phase suppress a zebrafish bmyb mutant" H.M. Stern, R. D. Murphey, J.L. Shepard, J. F. Amatruda, C. T. Straub, K.L. Pfaff, G. Weber, J.A. Tallarico, R.W. King, and L.I. Zon Nature Chemical Biology, 2005 1: 366-370. Pubmed

"Dissecting cell biology with chemical scalpels" Hathaway, N. and King, R.W. Curr. Opin. Cell Biol. 200517: 12 -19. Pubmed

"Adenoviral expression of reporter proteins for high-throughput cell-based screening" J. Hoyt and R.W. King In: Chemical Genomics: Reviews and Protocols. Zanders, E, Ed.., pp. 187-195 (Humana Press, Totawa N.J., 2005). Humana Press.

"Cytological profiling: Providing more haystacks for chemists' needles" J. Lorang and R.W. King  Genome Biology 20056:228.Pubmed

"Ubistatins inhibit proteasome-dependent degradation by binding the ubiquitin chain" Verma, R., Peters, R. D'Onofrio, M., Tochtrop, G.P., Sakamoto, K.M., Varadan, R., Zhang, M., Coffino, P., Fushman, D., Deshaies, R.J., and King, R.W. Science 2004306: 117-120. Pubmed 

Target identification strategies for chemical genetics." G. Tochtrop and R.W. King 2004J. Comb. Chem and High Throughput Screening 7:677-688. Pubmed

"Small molecule regulators of protein arginine methyltransferases" D. Cheng, N. Yadav, R.W. King, M.S. Swanson, E.J. Weinstein, M.T. Bedford J. Biol. Chem. 2004279: 23892-9. Pubmed


Education Publications

"Using cognitive load theory to evaluate and improve preparatory materials and study time for the flipped classroom" Fischer K, Sullivan AM, Cohen AP, King RW, Cockrill BA, Besche HC BMC Med Education 2023 23, 345. Full text Pubmed

Besche HC, Schwartzstein RM, King RW, Hoenig MP, Cockrill, B.A., 2022. A Step-by-Step Guide to Case-Based Collaborative Learning (CBCL). Springer International Publishing.

"Advanced integrated science courses: Building a skill set to engage with the interface of research and medicine" E.M. Miloslavsky, H.C. Besche, S.B. Calderwood, B.S. Chang, J.L. Dienstag, R.W. King, R.N. Mitchell, R.M. Schwartzstein, H. Thomas, E.M. Hundert, J.G. FLanagan Acad Med 2022  doi: 10.1097/ACM.0000000000004612 Pubmed

"The Harvard Medical School Pathways curriculum: Reimagining developmentally appropriate medical education for contemporary learners" R.M. Schwartzstein, J.L. Dienstag, R.W. King, B.S. Chang, J.G. Flanagan, H.C. Besche, M.P. Hoenig, E.M. Miloslavsky, K.M. Atkins, A. Puig, B.A. Cockrill, K.A. Wittels, J.L. Dalrymple, H. Gooding, D.A. Hirsh, E.K. Alexander, S.B. Fazio, E.M. Hundert, Pathways writing group. Acad Med 2020 doi: 10.1097/ACM.0000000000003270 Pubmed





Older Publications

Chemical Biology

"Roughing up smoothened: chemical modulators of Hedgehog signaling." R.W. King  2002 J. Biol. 1:8-12. PubMed

"New technologies for chemical genetics" L. A. Walling, N.R. Peters, J. Horn, R.W. King2001 J.Cell. Biochem. S37, 7-12. PubMed

"A one-bead, one-stock solution approach to chemical genetics: Part 2" P. Clemons, A. Koehler,  T.G. Sprigings,  D.R. Spring, R.W King, S.L. Scheriber, M.A. Foley Chem. Biol. 2001 8, 1183-1195. PubMed

"Small molecule inhibitor of mitotic spindle bipolarity identified in a phenotype-based screen" T.U. Mayer, T.M. Kapoor, S.J. Haggarty, R.W. King, S.L. Schreiber, T. J. Mitchison Science 1999 286, 971-974. PubMed

"Chemistry or biology: Which comes first after the genome is sequenced?" R.W. King Chem. Biol. 19996, R327-333. PubMed

"Dissecting cellular processes using small molecules: Identification of colchicine-like, taxol-like and other small molecules that perturb mitosis S.J. Haggarty, T.U. Mayer, D.T.  Miyamoto, R. Fathi, R.W. King,T.J. Mitchison, S.L. Schreiber Chem. Biol. 2000 7, 275-286. PubMed

"Stereochemistry of nucleophilic conjugate addition.  Addition of ethanol-d and 2-methyl-2-propanethiol-d to ethyl crotonate" J.R. Mohrig, S.S. Fu, R.W. King, R.W. Warnet, R.W., G. Gustafson J. Am. Chem. Soc. 1990 112, 3665-3667.

"Diastereoselectivity of enolate anion protonation. H/D exchange of b-substituted ethyl butanoates in ethanol-d" J.R. Mohrig, R.E. Rosenberg, J.W. Apostol, M. Bastienaansen, J.W. Evans, S.J. Franklin, C.D. Frisbie, S.S. Fu, M.L. Hamm, C. B. Hirose, D.A. Hundstad, T.L. James, R.W. King,C.J. Larson, H.A. Latham, D.A. Owen, K.A. Stein, R. Warnet J. Amer. Chem. Soc. 1997119, 479-486. 

Cell Cycle Control

"Anaphase is initiated by proteolysis rather than by the inactivation of MPF" S.L. Holloway, M. Glotzer, R.W. King, A.W. Murray Cell  1993 73, 1393-1402.  PubMed

 "Mitosis in transition" R.W. King, P.K. Jackson, M.W. Kirschner Cell  1994 79, 563-571. PubMed

"A 20S complex containing CDC27 and CDC16 catalyzes the mitosis-specific conjugation of ubiquitin to cyclin B" R.W. King, J.M. Peters, S. Tugendreich, M.Rolfe, P. Hieter, M.W. Kirschner Cell 1995 81, 279-288. PubMed

" Identification of a novel ubiquitin-conjugating enzyme involved in mitotic cyclin degradation" H. Yu, R.W. King, J.M Peters, M.W. Kirschner Curr. Biol. 1996 6, 455-466. PubMed

"Mutagenic analysis of the destruction signal of mitotic cyclins and structural characterization of ubiquitinated  intermediates" R.W. King, M. Glotzer, M.W. Kirschner Mol. Biol. Cell 1996 7, 1343-1357. PubMed

"Identification of BIME as a subunit of the anaphase-promoting complex" J.M. Peters, R.W. King, C. Hoog, M.W. Kirschner Science 1996 274, 1199-1201. PubMed

"How proteolysis drives the cell cycle" R.W. King, R.J. Deshaies, J. M. Peters, M.W. Kirschner Science 1996 274, 1652-1659. PubMed

Expression Cloning

"Systematic identification of mitotic phosphoproteins." P.T. Stukenberg, K.D. Lustig, T.J. McGarry, R.W. King, J. Kuang, M.W. Kirschner Curr. Biol. 1997 7, 338-348. PubMed

"Small pool expression screening: A  novel strategy for the identification of genes involved in cell cycle control, apoptosis, and early development." K.D. Lustig, P.T.  Stukenberg, T.J. McGarry, R.W. King, V.L. Cryns, P.E. Mead, L.I.  Zon, J. Yuan, M.W. Kirschner Meth. Enzym.1997 283, 83-99. PubMed

"Expression cloning in the test tube" R.W. King, K.D. Lustig, P.T. Stukenberg, T.J. McGarry, M.W. Kirschner Science 1997277, 973-974. PubMed